The purpose of this study was to evaluate the inhibitory effect of 03-carotene and PS-K on the oral transplanted tumor by the transplantation of Sarcoma 180 ascite tumor cells in the bone of incisor tooth germ region of the right mandible.
Three week-aged 56 male mice(ICR), weighing 20gm, were devided to 4 experimental groups and 2 control groups. Experimental group 1(12 mice) was transplanted 0.05ml Sarcoma 180 ascite tumor cells per a mouse with 25 Gauge syringe. Group 2(12 mice) was supplied orally 3 times per week with 1-2m1(3-carotene that was dissolved in soybeam milk after transplantation of Sarcoma 180. Group 3 (12 mice) was administered orally 3 times per week with 2m1 PS-K solution that was dissolved in soybeam milk at a dosage of lgm/200ntl after transplantation. Group 4(12 mice) administered 0-carotene and PS-K with the same method. Control group 5(6 mice) was administered 0-carotene and PS-K only and group 6(2 mice) was untreated control.
Five mice of each experimental group and 1 mouse of group 5 were sacrificed at 4, 6, 8, 10, 12, 14 days after experimentation. Then the mandible was removed, fixed in 10°N-Formalin and decalcified in 5% nitric acid. And embedded in paraffin; sectioned to 4? 6μm thickness, and stained with Hematoxylin and Eosin, Masson´s trichrome and Van Gieson by the usual method;
The results were as follows
1. Sarcoma 180 transplanted group revealed inflammatory reaction, degeneration and necrosis of transplanted region, located at 4 days, active malignancy of tumor cells at 6 days, infiltration into the alveolar bone and the periodontal membrane at 8 days, infiltrative proliferation into the periapical portion, adjacent muscular tissue, salivary gland and deep jaw bone at 10, 12 days.
2. The growth of the transplanted tumor cells was inhibited slightly in 0-carotene administered group, morelyinhibited in PS-K administered group and markedly inhibited in 0-carotene and PS-K combined group.
3. There are marked infiltration of lymphocytes due to exudative inflammatory reaction in the adjacent tissue to the transplanted tumor cells.
4. Control group were administered 0-carotene and PS-K only, revealed congestion of blood vessels at 4 days, edematous and hemorrhagic features at 8, 10 days.
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